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The drying of aqueous poly(ethylene oxide) (PEO) droplet on a substrate at different temperatures was studied. It was found that the contact line receded when the substrate was at a temperature above 60 ^oC. Different nucleation behavior and surface profiles of PEO films were found in different droplets drying processes. The rheological properties of aqueous PEO solutions were studied to understand the mechanism of contact line recession and micro-flow in drying aqueous PEO droplets. It was found that at low temperature, the contact line was static because of great viscous stress; while at high temperature, it receded because of great Marangoni force and the decrease of viscous stress. It was indicated that Marangoni convection was inhibited by the outward capillary flow and viscous stress at low temperature, whereas it became dominant at high temperature. Two types of mechanism for surface profiles and nucleation of PEO film from drying droplets are proposed, providing a theoretical guide for polymer solution application in oil and gas foam flooding technology.